Journal: Cell Regeneration

Article Title: Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture

doi: 10.1186/s13619-024-00220-y

Figure Lengend Snippet: The E6bS culture system does not affect the directed differentiation ability of stem cells. A Schematic diagram of cortical organoids induce. B Differentiation of cortical organoids from H9 cells cultured in E6bS medium. Scale bar: 50 µm. C Four-week differentiation of cortical organoids from H9 cells cultured in E6bS medium, showing cortical layers marked by TBR1 + , BRN2 + , and SOX2 + neural progenitor cells. Scale bar: 50 µm. D Schematic diagram of GABA precursor organoid induction. E Differentiation of GABA precursor organoids from H9 cells cultured in E6bS medium. Scale bar: 50 µm. F Four-week differentiation of GABA organoids from H9 cells cultured in E6bS medium, showing NKX2.1 + /TUJ1 + and SOX6 + /MAP2 + inhibitory progenitor cells and inhibitory interneurons. Scale bar: 50 µm. G Schematic diagram of heart-forming organoid induction. H Differentiation of heart-forming organoids from H9 cells cultured in E6bS medium, showing MYL7 + /cTnT + cardiomyocytes. Scale bar: 100 µm

Article Snippet: Heart-forming organoids were stained using the same method, with primary antibodies being cTnT (1:500, Thermo, MA512960) and MYL7 (1:500, proteintech, 17283–1-AP).

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: DMH1, a Novel BMP Small Molecule Inhibitor, Increases Cardiomyocyte Progenitors and Promotes Cardiac Differentiation in Mouse Embryonic Stem Cells

doi: 10.1371/journal.pone.0041627

Figure Lengend Snippet: (Left) DMH1 induces significant upregulation of transcription factor for cardiomyocyte commitment (Nkx2.5) compared with DMSO control. DM also induces Nkx2.5 expression, but the induction is less robust than DMH1. Results are presented as the average of three independent experiments. Expression levels shown are normalized to Day 0 expression levels. Error bars denote the S.E.M. (standard error of the mean). P-value is calculated using two-tailed Student’s t-test. (Right) Western blot showing increased Myl7 expression (∼19 kDa), a structural protein specific to cardiomyocytes, in CGR8 cells 8 days after DM or DMH1 induction. No Myl7 expression was observed after DMSO treatment. Alpha-tubulin (∼50 kDa) is shown as loading control. The relative intensities of Myl7 bands are normalized to alpha-tubulin and are shown at the bottom.

Article Snippet: The antibodies used were myosin heavy chain 7 (Myl7, clone B-10) from Santa Cruz Biotechnologies at a dilution of 1∶100, and alpha-tubulin (Clone 11H10, Cell Signaling Technologies) at a dilution of 1∶5000.

Techniques: Control, Expressing, Two Tailed Test, Western Blot